A blueprint of ectoine metabolism from the genome of the industrial producer Halomonas elongata DSM 2581T
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چکیده
منابع مشابه
A blueprint of ectoine metabolism from the genome of the industrial producer Halomonas elongata DSM 2581T
The halophilic γ-proteobacterium Halomonas elongata DSM 2581(T) thrives at high salinity by synthesizing and accumulating the compatible solute ectoine. Ectoine levels are highly regulated according to external salt levels but the overall picture of its metabolism and control is not well understood. Apart from its critical role in cell adaptation to halophilic environments, ectoine can be used ...
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The genome of the Halomonas elongata type strain DSM 2581, an industrial producer, was reevaluated using the Illumina HiSeq2500 technology. To resolve duplication-associated ambiguities, PCR products were generated and sequenced. Outside of duplications, 72 sequence corrections were required, of which 24 were point mutations and 48 were indels of one or few bases. Most of these were associated ...
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15 صفحه اولFunctional expression of the ectoine hydroxylase gene (thpD) from Streptomyces chrysomallus in Halomonas elongata.
The formation of hydroxyectoine in the industrial ectoine producer Halomonas elongata was improved by the heterologous expression of the ectoine hydroxylase gene, thpD, from Streptomyces chrysomallus. The efficient conversion of ectoine to hydroxyectoine was achieved by the concerted regulation of thpD by the H. elongata ectA promoter.
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ژورنال
عنوان ژورنال: Environmental Microbiology
سال: 2010
ISSN: 1462-2912
DOI: 10.1111/j.1462-2920.2010.02336.x